Uploaded all images (.JPG) taken thus far in to google drive folder: https://drive.google.com/drive/folders/0B_Kednl1tNImXzVhZUJzME9FOTA?usp=sharing
Measured two more tubes using ImageJ and uploaded the new .tiff images to this google drive folder: https://drive.google.com/drive/folders/0B_Kednl1tNImY0I3MzVnVE03QW8?usp=sharing.
Today I continued with taking pictures of the OA geoduck larvae for the morphology data collection. I have begun the process of uploading the pictures (.JPG) to a google drive folder where they can be accessed (https://drive.google.com/drive/folders/0B_Kednl1tNImXzVhZUJzME9FOTA?usp=sharing). I have a separate folder containing the images that have already been analyzed – M1 through M50 – and each larvae that has been measured is labeled (https://drive.google.com/drive/folders/0B_Kednl1tNImY0I3MzVnVE03QW8?usp=sharing).
Using a compound microscope and an iPhone camera, images were taken of geoduck larvae for the purpose of recording morphological measurements – length, width, and area – using ImageJ.
An example of an image used to take measurements:
Using ImageJ, the individual larvae are labeled using M#_L#. Where “M#” is the sample tube of larvae for the morphological data, and “L#” is the larvae number within a sample.
According to literature (Ferreira-Arrieta et al., 2015) it has been found that for the age of the larvae during the first several sample sets which I have measured, the average length is 110 +/- 30 µm (0.110 +/- 0.03 mm). This is close to the measurements that I have done.
A portion of the data thus far (measurements are in mm):
During sampling, hemolymph samples were not taken until geoduck specimen Geo-17. Hemolymph samples for the early stage geoduck had to be different than the tissue samples used in proteomics previously because there were no corresponding hemolymph samples. Therefore, the following geoduck hemolymph samples were set aside for proteomics:
With the following criteria for the stages: