Laura’s Notebook: May 27th, 2017

Here are some charts of the Oly larvae that I’ve collected & counted to date



Some documentation

Arrival inspection

Larval buckets:

  • Test bucket Check:
    • Not overflowing, despite not changing banjo yesterday.
    • T = 17.25 (T1)
    • pH = 7.88 (Durafet 2)
  • Aeration looks good
  • Algae concentration looks a bit weak – increased dosing rate from 60 -> 70
  • New larvae in following catchment buckets:
    • K-10 Low – a bit
    • HL10 Low – a ton!
    • SN-6 Low B – a lot
    • SN-10 Low B- a lot
    • NF-6 Low B – a bit
    • NF-6 Amb A- a bit
  • Maybe some in:
    • NF-10 Low B
    • NF-10 Low A
    • SN-6 Amb A
    • NF-10 Amb A
    • NF-6 Amb B

Need to buy:

  • 15 feet 1/2 pvc flex hose (for freshwater flush)
  • 1/4” tube connectors – straight
  • 1/8” tube connectors- Y’s and straight

Need to make:

  • Fresh water connection
  • Pipe with lots of holes for catch freshwater flush outflow
  • Microculch
  • Assemble setting tank silos

Tasks Today

  • Collect new larvae, stock, sample excess – DONE
    • This went smoothly, except that the banjo was left off group HL-10 low pH yesterday, so I therefore likely lost larvae. there still was quite a bit on the bottom of the bucket, but because of this likely fewer # of larvae in the bucket I added extra from today’s spawn.
  • Make freshwater connection – NOT COMPLETE, but I figured ou what I need to purchase
  • Clean top row broodstock & all components – DONE
  • Record location of top shelf broodstock on manifold – DONE
  • Change all banjos, drippers – DONE
  • Feed – DONE. used – 1/2 Ciso, 1/2 CGW
    • Increased algae capacity so I can take Sunday off:
      • 200L for larval tank
      • 200L for other tank
      • 100L for gigas
  • Image plates – NOT DONE

Discovered a flat tire on my bike, called an Uber- only $17, and he arrived within 15 minutes. Pretty sweet.

from The Shell Game

Laura’s Notebook: May 23rd – 26th, 2017

May 23rd

First full day of screenling larval buckets to assess survival, growth, and determine stocking numbers for next 3 days. Katie Davidson helped out, as did Steven.


  • Screen larvae for different size classes, count, re-stock buckets:
    • Screen through…. 140, 160, 180 … ?
      • Decided on 2 size classes, <180, >180. Screened through 160 today on first 2 groups, and nothing held; will screen through 180 next week. Only screened onto 100 today, counted live, and used that info to re-stock buckets.
    • Sample for counts & images
    • Sample for freezer
    • Stock buckets – method depends on # surviving larvae- Replace drippers – DONE
  • Replace banjos – DONE
  • Collect new larvae, count, stock, and sample excess
  • Measure dripper rate @ larval tank – NOT DONE
  • Collect water sample for algae counts from each dripper – NOT DONE
  • Record broodstock & larvae group locations on tables/manifolds – NOT DONE

May 24th

  • Arrival inspection
  • Image yesterday’s larvae – DONE
  • Change banjos – DONE
  • Change drippers – DONE
  • Collect & Sample for counts – DONE
  • Sample rest for -80 – DONE
  • Talk to Ryan about:
    • Larvae health – help trouble shooting?
      • Larvae looked sluggish when screening yesterday; this could simply be a response to the handling, however it is markedly different from the new larvae that are collected in a similar manner Ryan said that “lazy” larvae can be a sign of supersaturation of Nitrogen or Oxygen. He suggested raising the drippers out of the bucket to allow for degassing, and increasing the aeration. I did so.
    • Setting tank
      • Ryan showed me which silos I can use in the setting tanks outside; I checked with Joth (as he may have had plans for the tanks), but he said that I could use them through the month of June.
    • PSRF and other help while I’m at FHL?
      • Stuart has agreed to oversee the project
      • Jade and Dana can help out
      • Olivia to work Mondays (1x per week)

May 25th, 2017

Arrival Inspection:

  • Larval Table:
    • Aeration did not hold overnight.
    • Banojs very dirty, likely due to use of Tetraselmis in algae cocktail. I will request that PSRF not feed my larvae Tet.
    • I can see larvae swimming in water column, however there are a significant amount at the bottom of the bucket.
  • Larvae present in:
    • K-10 Low
    • HL-10 Ambient
    • K-10 Ambient
    • NF-6 Ambient A
    • NF-6 Low B
    • NF10 Ambient A


  • Collected Larvae, sampled and counted most (not al- need to finish)
  • Sampled immediately after collecting, then stocked. 6 buckets = 1.75 hrs by mysefl.
  • Recorded location of bottom shelf broodstock and sensors on manifold
  • Cleaned! the following took ~2 hrs
  • NF & SN broodstock thoroughly, including tubes, tube conenctors
  • Replaced all drippers, banjos & air stones
  • Set up 2 “test” buckets to measure T & pH in both larval systems, and to test whether banjos will clog over 2 days.
  • Sketched out possible June/July schedule coverage

May 26th, 2017

Arrival Inspection:

  • Larval Buckets
    • Aeration looks good
    • Very few bubbles accumulated on the inner walls of buckets- elevating the drippers and increasing aeration appeared to have been effective.
    • Banjos not consisently dirty; perhaps corresponding to the # larvae in bucket? Need to measure dripper rate on manifold to confirm equal flow rates.


  • NF-6 Ambient A group is foamy- male(s) likely spawning today
  • Water color looks light; increased algae dosing rate to 120 pulses/min

Larvae present in:

  • NF-6 Low B
  • NF-6 Ambient B
  • HL-10 Ambient
  • K-10 Low
  • K-6 Low
  • HL-10 Low (ID’d in afternoon)
  • SN-10 Low B (ID’d in afternoon)


  • Screen larval buckets, count, restock – 3 hrs for 16 buckets
    • Possibly use 2 different buckets per group, if needed – this was needed for SN-10 Low, & SN-10 Ambient
    • I was pleasantly surprised at the survival rate! Despite larvae consistently pooling at the bottom of the buckets, my survival rate for the SN & NF groups appears adequate (calculations TBD). The K group, which has been consistently stocked since Friday, had poorer survival rate. Need to refine data sheet to auto-calculate stocking #’s and survival rate
  • Collect new larvae, stock in buckets – 2 hrs
    • sample excess larvae & freeze
  • Image larvae from 5/23 & 5/24 & finish counts – 30 minutes -not done.
  • Daily maintenance: – 30 minutes – done.
    • Replace banjos
    • Replace drippers
    • Spray down filters
  • Clean top row broodstock if there’s time – 1 hr – not done.

from The Shell Game

Laura’s Notebook: May 22nd, 2017

Arrival Inspection

  • Larval table, top row:
    • Lots of bubbles on side of buckets; after speaking with PSRF this is likely a sign of supersaturated water (N or O).
    • Color very light – it appears that I have the same un-even algae distribution problem on this table.
    • Air stones not bubbling enough- I opened their valves a bit more and that worked. Seems like this is another depressurization issue. Not sure why.
    • Lots of larvae pooled on bottom of buckets- likely due to a combination of the above issues.


  • Larval table, bottom row:
    • banjos very dirty, but not clogged
    • air stones working well
    • algae concentration appears appropriate
  • Broodstock Table:
    • Things appear stable!
    • Very few bubbles on the sides of buckets
  • Larvae present in:
    • SN-10 amb B
    • NF-6 amb A
    • SN-6 low A
    • SN-6 amb B
    • NF-10 low A
    • K-6 low
    • K-10 amb

Today’s Tasks:

  • Moved all buckets that were on the top shelf of the larval table to the bottom shelf. Buckets that were on top shelf were: 11, 12, 13, 16.
    • Took photo of buckets 16 & 5 next to each other-obvious difference in algae concentration. img_8020
  • Cleaned banjos in larvae
  • Spoke with Ryan about June:
    • They do not know whether staff will be working weekends through June. If they don’t, then people w/o access cards/keys won’t have access to the lab. I asked whether, in that situation, PSRF would be able/willing to work my project on weekends. Ryan said maybe Jade or Stuart could- or perhaps one of them could run my project…
    • Ryan is going to talk to Stuart and consider whether Stuart or Jade could take the lead for my project while I’m at FHL. Will get an answer tomorrow.
  • Counted larvae collected yesterday
  • Imaged larvae collected yesterday
  • Collected new larvae, sampled, counted, stocked
    • Not too many larvae today. I’m streamlining this process, but currently takes a couple hours. Collection from today: 56999bb1-7cdc-4898-a156-e92e2c0fbaa4
  • Collected excess larvae in 2mL vials froze. Labeling scheme will be numerical:
    • Date, Oly, Vial # e.g. “5/22/2017 Oly 1-A”
    • I had saved excess larvae from 5/20 & 5/21, so I sampled those and the excess from today: fc88fe67-90d9-4733-bd5a-0f66f7f880fb
  • Determined how I should collect larval samples, and how frequently
    • I will collect larval samples daily when there are >10k excess larvae
    • Collection will occur after stocking buckets – I will save tripours with remaining larvae, then pull 2 samples each of 10k
  • Figure out how to fix the over saturation situation
    • Ryan said I could install small degassing columns on each bucket, that the water would flow through prior to entering the bucket. Hmm….
  • Figure out how to fix the algae concentration situation on the larval tank – not enough space to plumb algae input further back. Options could be:
    • Connect top and bottom manifold at other end to create a circular flow
    • Plumb in 2 pumps for each shelf

By the way, this is my banjo setup, which works very nicely in the quick-change: img_7926

To Do Tomorrow:

  • Replace drippers
  • Replace banjos
  • Measure dripper rate @ larval tank
  • Collect water sample for algae counts from each dripper – yikes!
  • Record broodstock & larvae group locations on tables/manifolds
  • Screen larvae for different size classes, count, re-stock buckets:
    • Screen through…. 140, 160, 180 … ?
    • Sample for counts & images
    • Sample for freezer
    • Stock buckets – method depends on # surviving larvae

To Do with time:

  • Make connection for freshwater – to do Thursday

from The Shell Game