May 23rd
First full day of screenling larval buckets to assess survival, growth, and determine stocking numbers for next 3 days. Katie Davidson helped out, as did Steven.
Tasks
- Screen larvae for different size classes, count, re-stock buckets:
- Screen through…. 140, 160, 180 … ?
- Decided on 2 size classes, <180, >180. Screened through 160 today on first 2 groups, and nothing held; will screen through 180 next week. Only screened onto 100 today, counted live, and used that info to re-stock buckets.
- Sample for counts & images
- Sample for freezer
- Stock buckets – method depends on # surviving larvae- Replace drippers – DONE
- Replace banjos – DONE
- Collect new larvae, count, stock, and sample excess
- Measure dripper rate @ larval tank – NOT DONE
- Collect water sample for algae counts from each dripper – NOT DONE
- Record broodstock & larvae group locations on tables/manifolds – NOT DONE
May 24th
- Arrival inspection
- Image yesterday’s larvae – DONE
- Change banjos – DONE
- Change drippers – DONE
- Collect & Sample for counts – DONE
- Sample rest for -80 – DONE
- Talk to Ryan about:
- Larvae health – help trouble shooting?
- Larvae looked sluggish when screening yesterday; this could simply be a response to the handling, however it is markedly different from the new larvae that are collected in a similar manner Ryan said that “lazy” larvae can be a sign of supersaturation of Nitrogen or Oxygen. He suggested raising the drippers out of the bucket to allow for degassing, and increasing the aeration. I did so.
- Setting tank
- Ryan showed me which silos I can use in the setting tanks outside; I checked with Joth (as he may have had plans for the tanks), but he said that I could use them through the month of June.
- PSRF and other help while I’m at FHL?
- Stuart has agreed to oversee the project
- Jade and Dana can help out
- Olivia to work Mondays (1x per week)
May 25th, 2017
Arrival Inspection:
- Larval Table:
- Aeration did not hold overnight.
- Banojs very dirty, likely due to use of Tetraselmis in algae cocktail. I will request that PSRF not feed my larvae Tet.
- I can see larvae swimming in water column, however there are a significant amount at the bottom of the bucket.
- Larvae present in:
- K-10 Low
- HL-10 Ambient
- K-10 Ambient
- NF-6 Ambient A
- NF-6 Low B
- NF10 Ambient A
Tasks:
- Collected Larvae, sampled and counted most (not al- need to finish)
- Sampled immediately after collecting, then stocked. 6 buckets = 1.75 hrs by mysefl.
- Recorded location of bottom shelf broodstock and sensors on manifold
- Cleaned! the following took ~2 hrs
- NF & SN broodstock thoroughly, including tubes, tube conenctors
- Replaced all drippers, banjos & air stones
- Set up 2 “test” buckets to measure T & pH in both larval systems, and to test whether banjos will clog over 2 days.
- Sketched out possible June/July schedule coverage
May 26th, 2017
Arrival Inspection:
- Larval Buckets
- Aeration looks good
- Very few bubbles accumulated on the inner walls of buckets- elevating the drippers and increasing aeration appeared to have been effective.
- Banjos not consisently dirty; perhaps corresponding to the # larvae in bucket? Need to measure dripper rate on manifold to confirm equal flow rates.
Broodstock:
- NF-6 Ambient A group is foamy- male(s) likely spawning today
- Water color looks light; increased algae dosing rate to 120 pulses/min
Larvae present in:
- NF-6 Low B
- NF-6 Ambient B
- HL-10 Ambient
- K-10 Low
- K-6 Low
- HL-10 Low (ID’d in afternoon)
- SN-10 Low B (ID’d in afternoon)
Tasks:
- Screen larval buckets, count, restock – 3 hrs for 16 buckets
- Possibly use 2 different buckets per group, if needed – this was needed for SN-10 Low, & SN-10 Ambient
- I was pleasantly surprised at the survival rate! Despite larvae consistently pooling at the bottom of the buckets, my survival rate for the SN & NF groups appears adequate (calculations TBD). The K group, which has been consistently stocked since Friday, had poorer survival rate. Need to refine data sheet to auto-calculate stocking #’s and survival rate
- Collect new larvae, stock in buckets – 2 hrs
- sample excess larvae & freeze
- Image larvae from 5/23 & 5/24 & finish counts – 30 minutes -not done.
- Daily maintenance: – 30 minutes – done.
- Replace banjos
- Replace drippers
- Spray down filters
- Clean top row broodstock if there’s time – 1 hr – not done.
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