Sean’s Notebook: Canu running, figuring out Racon and what it needs next.
So I got Racon, a consensus caller installed, and it looks like it requires mapping position information. The Racon developers suggested Minimap for that purpose. I installed Minimap on Emu so I could run it on the filtered reads while Canu is running on Hyak.
It looks like the general process will be as follows
- Canu to make preliminary contigs
- Map Raw reads to contigs with Minimap
- Consensus call with Racon
- Repeat the above steps ~ 3 times (that seems to be a common number)
- Map the raw Illumina reads to the final Racon output with samtools
- Use Pilon to polish with the mapped .sam files.
- End with an assembly based on PacBio reads, and finished with Illumina, the opposite of the Platanus/Redundans workflow.
We’re currently on step 1, so this might take a little while…
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