Kaitlyn’s Notebok: Gene enrichment of unique proteins

I grouped proteins that had 0 abundance on day 1 based on the number of days they were abundant and ran it through CompGO for biological processes at 0.1. To see if there was a differences in the number of proteins expressed in each group, I made a small table. All values were similar. Highlighted values had p-values of at least 0.1.

I think it’s interesting that all silos had enrichment with proteins that had abundance for only 1 day. All silos had peptidyl-tyrosine-dephosphorylation or “the removal of phosphoric residues from peptidyl-O-phospho-tyrosine to form peptidyl-tyrosine” at p-values greater than 1E-1.


Silo 2- 1 day of protein abundance:

Silo 3- 1 day of protein abundance:

Silo 9- 1 day of protein abundance:

Other enriched processes are:

Silo 2-  cellular response to retionoic acid (6 days),

Silo 3-  intracellular protein transport (4 days) and maturation of SSU-rRNA from tricistronic rRNA transript (5 days),

and Silo 9: negative regulation of endopeptidase activity (7 days). This can be viewed below in respective order.

Silo 2- 6 days of protein abundance:

Silo 3- 4 and 5 days of protein abundance respectively:

Silo 9- 7 days of protein abundance:

Katie’s Notebook: Staging finished! 7/10/17

I finished staging the pictures Grace took of the slides made after overwintering and entered all my data into an excel sheet today! I added all of the sex and maturity data for ever oyster photographed with visible gonad tissue. I then created some pivot tables to get some total distribution information. These are shown in the tables and pie charts in the second picture. Finally I sorted all of the data by sex and maturity stage and totaled how many of each there were in every experimental category. Mostly just played around with different ways to combine the numbers!

Note: Data from the pictures Grace took of the slides from this winter are labeled “post-temp” and data from the pictures I took of the slides from April are labeled as “post-OA”.
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The original excel document is in my folder on OWL.