Sam’s Notebook: Genome Annoation – Olympia oyster genome annotation results #01

0000-0002-2747-368X

Yesterday, I annotated our Olympia oyster genome using WQ-MAKER in just 7hrs!.

See that link for run setup and configuration.

Roberto’s Notebook: DNA quality

The concentration of DNA samples extracted from gill tissue had the values:
Screen Shot 2018-08-07 at 5.25.58 PM

Using 100 ng, the integrity of DNA was observed in agarose gels.
Screen Shot 2018-08-07 at 5.19.04 PM

The samples 05R1, 35R1, 52R1 and 59R1 do not present a good DNA integrity and samples 9 to 17 have small RNA presence.
The samples 1-4, 1-5, 1-6, 13-3, 13-4 and 13-5 showed in table, were extracted using the E. Z. N. A. Mollusc DNA Kit and were corroborated in agarose gel. The samples had a good DNA integrity (picture not shown). The rest of DNA samples were extracted using the salts protocol.