Dissections
I spent some time going through the measurements made during dissection to ensure everything was correct. There were quite a few errors or over sites that needed fixing so I’m glad I took the time to do this.Everything is finalized in the spreadsheet now (linked in my previous two posts). I highlighted any inaccuracies, missing measurements or incomplete recordings.
I also wanted to make a note here of who did what one each day of the dissections:
9/6/18: Fidalgo Bay- Duncan cleaned and measured oysters while I recorded measurements he called. Emily and Micah did large pouches and dissections.
9/7/18: Case Inlet- Emily and Micah separated pouches and were about halfway through measurements and dissections when I arrived and measured and recorded small oyster bags.
9/8/18: Port Gamble- Emily and Micah did the large pouches and dissections while I did the small pouches. Corey and Eliza arrived to help but we were mostly done. They helped measure and record a few bags though!
9/9/18: Skokomish- It was just Emily, Micah and I today. They did large bags and dissections while I did small bags again.
All dissections were done in 213 and tissue samples were flash frozen in liquid nitrogen before being out in the -80C. Digital calipers were used the first two days and the last two days both digital and an analog caliper was used. The scale in 209 was used for mass.
Pouch/length datasheet:




Tissue sampling datasheets:
Geoduck Measurements
I’m starting to measure the geoduck from outplanting. One horizontal line on the grid is 18mm long. I use this for the set measurement. However, each group will need this redone because the distance and zoom between images is variable.
I just started measurements, but I am saving ROI sets as I did with previous ImageJ measurements I’ve done.