I spent some time going through the measurements made during dissection to ensure everything was correct. There were quite a few errors or over sites that needed fixing so I’m glad I took the time to do this.Everything is finalized in the spreadsheet now (linked in my previous two posts). I highlighted any inaccuracies, missing measurements or incomplete recordings.
I also wanted to make a note here of who did what one each day of the dissections:
9/6/18: Fidalgo Bay- Duncan cleaned and measured oysters while I recorded measurements he called. Emily and Micah did large pouches and dissections.
9/7/18: Case Inlet- Emily and Micah separated pouches and were about halfway through measurements and dissections when I arrived and measured and recorded small oyster bags.
9/8/18: Port Gamble- Emily and Micah did the large pouches and dissections while I did the small pouches. Corey and Eliza arrived to help but we were mostly done. They helped measure and record a few bags though!
9/9/18: Skokomish- It was just Emily, Micah and I today. They did large bags and dissections while I did small bags again.
All dissections were done in 213 and tissue samples were flash frozen in liquid nitrogen before being out in the -80C. Digital calipers were used the first two days and the last two days both digital and an analog caliper was used. The scale in 209 was used for mass.
Tissue sampling datasheets:
I’m starting to measure the geoduck from outplanting. One horizontal line on the grid is 18mm long. I use this for the set measurement. However, each group will need this redone because the distance and zoom between images is variable.
I just started measurements, but I am saving ROI sets as I did with previous ImageJ measurements I’ve done.