After confirming Ronit’s DNased RNA was free of gDNA, I quantified the DNased RNA from 20181115 using the Roberts Lab Qubit 3.0 and the Qubit hsRNA Assay.
Used 1uL of each sample.
After confirming Ronit’s DNased RNA was free of gDNA, I quantified the DNased RNA from 20181115 using the Roberts Lab Qubit 3.0 and the Qubit hsRNA Assay.
Used 1uL of each sample.
On 11/8, I finished up the RNA extraction for the final 16 samples of the desiccation + elevated temp. exposure (D05, D06, D07, D08, D15, D16, D17, D18, T05, T06, T07, T08, T15, T17, T18). I also ran the Qubit assay for 24 samples (D03, D04, D05, D06, D07, D08, D13, D14, D15, D16, D17, D18, T03, T04, T05, T06, T07, T08, T13, T14, T15, T16, T17, T18). 4 samples (D06, T05, T15, T17) had RNA concentrations above the limit of quantification, so I will have to dilute those samples and re-run the Qubit assay. Described below is the protocol for both the RNA extraction wrap-up and the Qubit assay:
RNA Extraction Wrap-Up:
RNA Quantification (Qubit)