I’ve come full circle with my data from the Olympia oyster adult OA exposure project from 2017. After agonizing over the data for months (…years?), I’m moving forward with population-specific analysis, focusing on the effect of low pH on gonad & fecundity, with some minor findings regarding the offspring. Until a couple days ago I was all set to just use data from the 6C pre-treatment temperature groups (overwintered at 6C), but since we are now looking at population specific effects I may want to include the 10C groups (overwintered at 10C). The reasons to include both temperature groups are a) very simliar gonad results in both groups, b) more spawning data which support the population-specific reproduction theme, and c) I can refer to this paper when I write my QuantSeq paper (since RNA from larvae was from 10-low pH and 6-amb pH). If I go this route I will not analyze the 10C survival/growth data.
I’ve prepared some new figure options, particularly for the gonad histology data:
6C gonad data only, showing dominant stage, sex, and example gonad tissue
Gonad stage before pH treatment (n=54) and after 52 days in ambient pH (7.82±0.02, n=39) and low pH (7.31±0.02, n=39) for all populations combined. Gonad differed significantly between pH treatments (𝝌2=9.79, p=0.032), and between pre-treatment and ambient pH (𝝌2=6.61, p=0.146)