Checked DNA integrity of the Crassostrea virginica mantle gDNA I isolated on 20181114 in preparation for MBD enrichment. Detailed sample info and sample processing (including calculations for MBD enrichment using the MethylMiner Kit) are here (Google Sheet):
Loaded 100ng of each sample on a 0.8% agarose, 1X Low TAE gel with ethidium bromide. Each sample was mixed with 3uL of 6x loading dye and brought up to 18uL with nuclease-free H2O. Gel was run for ~2hrs @ 100V. Used two ladders in a (failed) attempt to accurately assess gDNA size:
The idea being that the HighRange ladder would handle high integrity gDNA molecular weight, while the DNA Ladder Mix would handle any degraded DNA and/or RNA present.