Sam’s Notebook: MBD Enrichment – C.virginica Sheared Mantle DNA from 20190306

Last week I performed MBD enrichment on 12 of the 24 Lotterhos C.viriginica mantle DNA on samples I had sheared on 20190306. I proceeded with MBD enrichment using the MethylMiner Kit (Invitrogen) on the 12 remaining samples of the 24. I followed the manufacturer’s protocol for input DNA >1ug – 10ug with the following modifications:

  • 2500ng of input DNA used
  • discarded washes post-DNA-binding
  • performed high-salt elution

The full sample list and calculations are here (Google Sheet):

Will proceed with ethanol precipitations of all 24 samples.

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