Last week I performed MBD enrichment on 12 of the 24 Lotterhos C.viriginica mantle DNA on samples I had sheared on 20190306. I proceeded with MBD enrichment using the MethylMiner Kit (Invitrogen) on the 12 remaining samples of the 24. I followed the manufacturer’s protocol for input DNA >1ug – 10ug with the following modifications:
- 2500ng of input DNA used
- discarded washes post-DNA-binding
- performed high-salt elution
The full sample list and calculations are here (Google Sheet):
Will proceed with ethanol precipitations of all 24 samples.