Sam’s Notebook: RNA Isolation and Quantification – Crab Hemolypmh Using Quick-DNA-RNA Microprep Plus Kit

In the continuing struggle to isolate RNA from the Chionoecetes bairdi hemolymph preserved in RNAlater, Pam managed to find the Quick-DNA-RNA Microprep Plus Kit (ZymoResearch) as a potential option. We received a free sample of the kit and so I gave it a shot. Grace pulled 10 samples she’d previously used to isolate RNA (and was unable to get anything out of virtually all of them using the RNeasy Plus Micro Kit (Qiagen)) for me to try out this new kit:

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I’ve included an image of the sample tubes, due to the fact that they are colored, and I can’t remember the significance (insignificance?) of this. Want to make sure that is documented in case it’s important.

10 samples used for RNA isolation in ice bucket