Kaitlyn’s notebook: 20190419 Pt. Whitney geoducks

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Tank 6:

Tank 6

Tank 5:

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Tank 4:

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Juveniles overwintered in heath stacks:

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Some signs of buildup/infestation:

  • The first geoduck is alive.
  • The second image contains two shells and a dead geoduck.

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KCL Priming experiment:

Additionally, Sam worked on an experiment to determine the optimum time for KCL egg priming. These are some important points about the experiment (more info is on Slack):

  • 6 x 10 experiment
  • Screened fertilized eggs (~1500) and took sample 1 hour post-fertilization from screen (concentrated sample).
  • Added eggs into glass bottle (0.3 um filtered seawater)
  • Bottles stored in wine cooler

Sam screening bottles:

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Bottles were discarded because we did not see many D-hinge larvae:

  • Sam looked and believed he saw some evidence of fertilization (formation of polar bodies) in samples taken 1 hour post-fertilization and will looks through those samples instead.

 

Kaitlyn’s notebook: Geoduck heath tray setup

Elevated header tank:IMG_20190419_085330

Dripper and outflow pipe to heath trays:

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Heath stack 1:

  • Red are elevated CO2 lines, blue is ambient.
  • Nozzles on the line control flow into the tray.
  • Boards are covering the top trays.20190419_090944

Elbow pipes on heath trays:

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Accessing the water lines:

  • It is easy to pull on the water line system when pulling out individual trays. There is access to the back of the trays so that the water line can be removed so the tray can be pulled out.
  • However, this does make the trays stick out into the walkway. We zip-tied poles onto the edge of the stack in front of the elbow pipes to prevent people from directly hitting anything.
  • Also, the elevated water treatment has some bubbles that are generated in the line.

Line from algal header tank:

  • Sam added a thinner line (connected below the green zip tie) to improve flow.
  • The line runs to an algal header tank in the breezeway that is filled in the mornings and feeds everything in this room. The tank runs out in the evening.

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Juvenile geoduck in H1T1:

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For comparison, here are 5 week old industry geoduck:

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Interestingly, juveniles were crawling up the side in one industry tank:

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