# Check on heath stack setup

• food still dicey, sometimes the food is unevenly distributed.
• ordered a new pump head today and they are sending some sample tubing to see if it will solve the problem (see below for more details).
• animals looked good but some algal floculation
• counted about 5-7 animals in ~15 mL water/sand grab from second tray in heath stack (“extra” juveniles from variable low pH parents)
• need to clean mesh next week
• need to shuffle trays around

# Algal counts

• Matt recommended setters should get a concentration of 100K/mL, but not more than that.
• Inflow algal counts direct from the feeding tube were:
• H2 (ambient): 4.73 x 10^6 cells/mL
• H1 (ambient/low pH): 4.8 x 10^6 cells/mL
• Outflow algal counts direct from the feeding tube were:
• H2 (ambient): 5.3 x 10^5 cells/mL
• H1 (ambient): 3.8 x 10^5 cells/mL
• Flow rate of the feeding tube is 10mL/13 seconds = 0.77mL/second = 46.2 mL/minute = 66,528 mL / day = 35,260 M cells/day
• FAO manual recommends 0.4mg dry algae per mg spat per week – 10^6 Iso cells = 0.02mg, so 20M cells/week or 2.857M cells/day
• Setter weight ~= 0.07mm^3 = 0.07mg? – H2 has ~ 52000 animals = 3.640 mg spat – H1 ambient has ~ 60,000 animals = 4.2mg spat

Conclusion

• Ambient should be getting ~ 22.4M cells/day (2.857M cells/day * 7.84 mg total spat)
• They are getting about 1.5X as much

Solutions

# Water chem

Kaitlyn and Eileen ran TA on H1 water samples. TA data here and discrete chem data here

• Still need to run poisoned samples from H1T5-T8 from 4/19 and from 5/2. We can do this next week.

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