Laura’s Notebook: September, 2019 Goals

Review of last month’s goals

QuantSeq:

  • Quantify remaining RNA with Qubit – complete
  • Assess RNA quality via Bioanalyzer on subset of samples – complete
  • Concentrate dilute samples. Goal is 500 ng in 5 uL – not done
  • Acquire all materials for QuantSeq library prep – complete
  • Test protocol on 8 samples (“extra” samples). – in progress

Degree:

  • Identify and reach out to potential new committee members – complete. GSR is Jen Ruesink!
  • Read Jackie’s stack of papers, take notes and organize using Evernote – in progress!
  • Get study lists from Rick & Steven – in progress. Received from Rick

Manuscripts:

  • Finish simplifying O. lurida temperature/food methods, results and discussion – complete, sent to Steven for review
  • sBegin revising, or at least organize thoguhts on, Polydora MS as per discussion with Chelsea & Julieta – not started!

PCSGA:

  • Review presentation from Aquaculture 2019 for PCSGA. – in progress
  • Measure larvae from 2017 OA/T study – complete

## New Goals

### QuantSeq

  • Troubleshoot issues with trial run :/
  • Make headway on preparation of actual samples

### Degree:

  • Continue reading to prep for Quals – need to pick up speed
  • Get reading list from Steven
  • Meet with Jen
  • Meet with Krista & Mac re: GRIP
  • Can I submit dissertation proposal?

Manuscripts

  • Oly temp/food
    • Revise methods -> discussion based on Steven’s feedback
    • Write intro
  • Polydora -> redraft (!)
  • Maybe get reviewer comments from Ecological Applications? (Not to-do, but something to anticipate)

### Other

  • NSA quarterly newsletter piece
  • Present @ PCSGA
  • Figure out Jackie’s scope calibration situation …

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