Today I did some more extractions, but moved on to day 12. I did a mix of all groupings (temperature and infection statuses). All extracted samples had detectable RNA! Details in post. Additionally, I’ll give a summary table of what all I have extracted so far.
Samples extracted today:
FRP |
trtmnt_tank |
sample_day |
infection_status |
maturity |
tube_number |
---|
6137 |
ambient |
12 |
1 |
I |
301 |
6122 |
ambient |
12 |
1 |
I |
325 |
6125 |
ambient |
12 |
1 |
I |
303 |
6176 |
ambient |
12 |
0 |
M |
329 |
6179 |
ambient |
12 |
0 |
M |
315 |
6213 |
ambient |
12 |
0 |
I |
310 |
6104 |
cold |
12 |
0 |
I |
259 |
6106 |
cold |
12 |
0 |
M |
241 |
6118 |
cold |
12 |
1 |
I |
240 |
6120 |
cold |
12 |
1 |
I |
248 |
6126 |
cold |
12 |
1 |
I |
201 |
6191 |
cold |
12 |
0 |
I |
227 |
6148 |
cold |
12 |
1 |
I |
213 |
6149 |
cold |
12 |
1 |
I |
226 |
6151 |
cold |
12 |
1 |
I |
243 |
6242 |
warm |
12 |
0 |
M |
377 |
6249 |
warm |
12 |
1 |
I |
279 |
6250 |
warm |
12 |
1 |
I |
294 |
6251 |
warm |
12 |
1 |
I |
376 |
6254 |
warm |
12 |
0 |
I |
296 |
6259 |
warm |
12 |
0 |
M |
281 |
6260 |
warm |
12 |
0 |
M |
374 |
6264 |
warm |
12 |
0 |
I |
268 |
6265 |
warm |
12 |
0 |
I |
282 |
Sample prep and extraction
I did everything the same as what I’ve done for the past two extractions (1 and 2). No obvious mishaps or mistakes as far as I know.
Results:
[Raw qubit] [Google sheet qubit]
qubit_tube_conc_ng.ml |
original_sample_conc_ng.ul |
sample_vol_ul |
dilution_factor |
tube_number |
extraction_method |
ul_sample-used |
elution_vol_ul |
total-yield_ng |
---|
244 |
24.4 |
2 |
100 |
282 |
Zymo_microprep |
35 |
15 |
317.2 |
152 |
15.2 |
2 |
100 |
268 |
Zymo_microprep |
35 |
15 |
197.6 |
183 |
18.3 |
2 |
100 |
374 |
Zymo_microprep |
35 |
15 |
237.9 |
231 |
23.1 |
2 |
100 |
281 |
Zymo_microprep |
35 |
15 |
300.3 |
84.9 |
8.49 |
2 |
100 |
296 |
Zymo_microprep |
35 |
15 |
110.37 |
84.7 |
8.47 |
2 |
100 |
376 |
Zymo_microprep |
35 |
15 |
110.11 |
52.3 |
5.23 |
2 |
100 |
294 |
Zymo_microprep |
35 |
15 |
67.99 |
355 |
35.5 |
2 |
100 |
279 |
Zymo_microprep |
35 |
15 |
461.5 |
223 |
22.3 |
2 |
100 |
377 |
Zymo_microprep |
35 |
15 |
289.9 |
23 |
2.3 |
2 |
100 |
243 |
Zymo_microprep |
35 |
15 |
29.9 |
262 |
26.2 |
2 |
100 |
226 |
Zymo_microprep |
35 |
15 |
340.6 |
340 |
34 |
2 |
100 |
213 |
Zymo_microprep |
35 |
15 |
442 |
180 |
18 |
2 |
100 |
227 |
Zymo_microprep |
35 |
15 |
234 |
62.7 |
6.27 |
2 |
100 |
201 |
Zymo_microprep |
35 |
15 |
81.51 |
134 |
13.4 |
2 |
100 |
248 |
Zymo_microprep |
35 |
15 |
174.2 |
216 |
21.6 |
2 |
100 |
240 |
Zymo_microprep |
35 |
15 |
280.8 |
130 |
13 |
2 |
100 |
241 |
Zymo_microprep |
35 |
15 |
169 |
317 |
31.7 |
2 |
100 |
259 |
Zymo_microprep |
35 |
15 |
412.1 |
273 |
27.3 |
2 |
100 |
310 |
Zymo_microprep |
35 |
15 |
354.9 |
178 |
17.8 |
2 |
100 |
315 |
Zymo_microprep |
35 |
15 |
231.4 |
29 |
2.9 |
2 |
100 |
329 |
Zymo_microprep |
35 |
15 |
37.7 |
274 |
27.4 |
2 |
100 |
303 |
Zymo_microprep |
35 |
15 |
356.2 |
311 |
31.1 |
2 |
100 |
325 |
Zymo_microprep |
35 |
15 |
404.3 |
289 |
28.9 |
2 |
100 |
301 |
Zymo_microprep |
35 |
15 |
375.7 |
Summary table of what I have extracted so far:
from Grace’s Lab Notebook https://ift.tt/2q3zf4h
via IFTTT