Steven was recently contacted by Marinelli Shellfish Company to see if we could help them determine if some oysters they had were Crassostrea gigas (Pacific oyster) or Crassostrea sikamea (Kumamoto). Steven knows of a paper with primer sequences to use with qPCR for this specific determination.
They sent ~12 of:
- known Crassostrea gigas
- known Crassostrea sikamea
We collected mantle tissue from 12 of each group. Samples were labeled in the following fashion:
- C.gigas 1191-SS ## (known Crassostrea gigas)
- C.sikamea CA5SS ## (known Crassostrea sikamea)
- C.sikamea 1191-SS ## (unknown)
Samples were stored at -80oC in Rack 2, Column 4, Row 5:
NOTE: Only 11 samples were collected for C.sikamea CA5SS.