- 1712 (Pg_DN51983i8_1471)
- 1711 (Pg_DN51983i8_1347)
Positive control used was same pooled gonad cDNA used succesffully on 20181206 to test the assay. Due to sample limitations, positive control was not run in duplicate!
All qPCR reactions were run in duplicate. See qPCR Report (Results section below) for plate layout, cycling params, etc.
qPCR Master Mix calcs (Google Sheet):
NOTE: I actually ended up running this twice (55oC and 60oC)due to poor melt curves when run at 55oC anneal.