Grace’s Notebook: Extract RNA from 24 samples – Day 9, 12 infected, 12 uninfected; still need to run on Qubit

Today I extracted RNA from 24 samples. Day 9, 12 infected and 12 uninfected. I did not run them on the qubit yet. Will do later this week.

Samples I used:

FRP trtmnt_tank day infection_status maturity tube_number
6251 NA 9 1 I 167
6263 NA 9 1 I 179
6262 NA 9 1 I 168
6261 NA 9 1 I 164
6256 NA 9 1 I 146
6257 NA 9 1 I 138
6266 NA 9 1 I 156
6255 NA 9 1 I 143
6240 NA 9 1 I 134
6141 NA 9 1 I 123
6128 NA 9 1 I 112
6118 NA 9 1 I 87
6268 NA 9 0 I 97
6260 NA 9 0 M 136
6259 NA 9 0 M 139
6254 NA 9 0 I 69
6253 NA 9 0 I 25
6248 NA 9 0 I 49
6242 NA 9 0 M 39
6238 NA 9 0 M 9
6235 NA 9 0 I 35
6234 NA 9 0 I 2
6232 NA 9 0 M 52
6230 NA 9 0 M 22

Method

Pretty much did everything the same as this post: Oct 25, 2019

New thing: eluted RNA in 15ul of TE

Where are they now?

Extracted RNA are in -80C (Rack 3, Column 2, Row 3)

DNA are in the fridge in FTR 209 – box 2 of C. bairdi DNA.

Remaining hemolymph samples were placed in Box 2 of the Day 9 (green caps) pellet box (Rack 8, Col 2, Row 5).

Next steps:

Run 2ul on Qubit using RNA HS Kit.

These might help with bulking up the pooled for NWGC that now no longer make the cut-off for their requirements after I attempted to concentrate the RNA (see post: 01/10/2020).

from Grace’s Lab Notebook https://ift.tt/30muOQd
via IFTTT