Sam’s Notebook: DNA Isolation and Quantification – C.bairdi Hemolymph Pellets in RNAlater

Isolated DNA from the following 23 samples:

  • 6128_112_9
  • 6204_114_9
  • 6141_123_9
  • 6245_126_9
  • 6240_134_9
  • 6260_136_9
  • 6257_138_9
  • 6259_139_9
  • 6258_140_9
  • 6255_143_9
  • 6256_146_9
  • 6265_155_9
  • 6266_156_9
  • 6261_164_9
  • 6120_165_9
  • 6251_167_9
  • 6262_168_9
  • 6243_173_9
  • 6263_179_9
  • 6264_180_9
  • 6200_208_12
  • 6204_252_12
  • 6190_256_12

Isolated DNA using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.

  • Used 35uL from each RNAlater/hemocyte slurry.
  • Mixed with equal volume of H2O (35uL).
  • Retained DNA on the Zymo-Spin IC-XM columns for isolation after RNA isolation.
  • DNA was eluted in 15uL H2O

DNA was quantified on the Roberts Lab Qubit 3.0 using the 1x DNA High Sensitivity Assay (Invitrogen), using 2uL of each sample.

Two samples were too concentrated, so those two samples were re-quantified using 1uL of sample.

Sam’s Notebook: RNA Isolation and Quantification – C.bairdi Hemolymph Pellets in RNAlater

TL;DR – Recovered absolutely no RNA from any sample! However, I did recover DNA from each sample.

Isolated RNA from the following 23 hemolymph pellet samples:

  • 6128_112_9
  • 6204_114_9
  • 6141_123_9
  • 6245_126_9
  • 6240_134_9
  • 6260_136_9
  • 6257_138_9
  • 6259_139_9
  • 6258_140_9
  • 6255_143_9
  • 6256_146_9
  • 6265_155_9
  • 6266_156_9
  • 6261_164_9
  • 6120_165_9
  • 6251_167_9
  • 6262_168_9
  • 6243_173_9
  • 6263_179_9
  • 6264_180_9
  • 6200_208_12
  • 6204_252_12
  • 6190_256_12

Isolated RNA using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.

  • Used 35uL from each RNAlater/hemocyte slurry.
  • Mixed with equal volume of H2O (35uL).
  • Retained DNA on the Zymo-Spin IC-XM columns for isolation after RNA isolation.
  • Performed on-column DNase step.
  • RNA was eluted in 15uL H2O

RNA was quantified on the Roberts Lab Qubit 3.0 using the RNA High Sensitivity Assay (Invitrogen), using 2uL of each sample.