Sam’s Notebook: RNA Isolation and Quantification – C.bairdi Hemocyte Pellets in RNAlater

Isolated RNA from the following hemolymph pellet samples:

  • 6202_337_12
  • 6142_474_26
  • 6144_477_26
  • 6122_489_26
  • 6176_490_26
  • 6136_491_26
  • 6137_500_26
  • 6125_503_26
  • 6145_505_26
  • 6131_507_26
  • 6159_508_26
  • 6206_509_26
  • 6150_511_26
  • 6161_512_26
  • 6115_514_26
  • 6179_515_26
  • 6172_517_26

Isolated RNA using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.

  • Used 35uL from each RNAlater/hemocyte slurry.
  • Mixed with equal volume of H2O (35uL).
  • Retained DNA on the Zymo-Spin IC-XM columns for isolation after RNA isolation.
  • Performed on-column DNase step.
  • RNA was eluted in 15uL H2O

RNA was quantified on the Roberts Lab Qubit 3.0 using the RNA High Sensitivity Assay (Invitrogen), using 2uL of each sample.

RNA was stored in the [-80o</sup>C freezer](http://b.link/srlab-80C) in Shellfish Box #8.