Isolated DNA from 56 samples (see Qubit spreadsheet in “Results” below for sample IDs) using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.
These samples were from RNA isolations on the following dates:
Brief rundown of method:
- Used 35uL from each RNAlater/hemocyte slurry.
- Mixed with equal volume of H2O (35uL).
- Retained DNA on the Zymo-Spin IC-XM columns at 4oC for isolation after RNA isolation.
- DNA was eluted in 15uL H2O
DNA was quantified on the Roberts Lab Qubit 3.0 using the 1x DNA High Sensitivity Assay (Invitrogen), using 1uL of each sample.