Grace’s Notebook: Submitted 6 pooled crab samples to NWGC for QC

Today I submitted 6 pooled samples of Crab RNA to NWGC for QC. After they run QC, they’ll let us know what our sequencing options are.

GitHub Issue: #798

Pooled the samples originally on Nov 22nd, 2019

After pooling, I realized I had to have samples concentrated to NWGC minimum requirement of 50 ng/ul… so I attempted to concentrate them using a kit from Zymo after it arrived in the mail.

Attempt at concentrating was unsuccessful becuase I didn’t do the correct volume of RNA Binding Buffer (post: here).

Sam recommended I contact NWGC and ask if we can still get some sequencing done even though our samples are not at their requirements, which brought us to today!

I put the 6 pooled samples in a plate that was provided by Jeff Weiss at NWGC, and walked them over there.

They will do QC testing, and then let us know what our sequencing options are afterwards.

Here’s manifest of what I submitted today:

Plate Well Location Investigator Sample ID Additional Sample ID Family Number Replacement Sex Date of Birth Organism Race Concentration (ng/uL) Volume (uL) RNA Quality Score Sample Source Type of Sample Suspended In Extraction Method Certified for dbGaP dbGaP ID Investigator Last Name
(Required) (Required) (Required) (Required) (Required) (Required if RNAseq) (Required) (Required) (Required)
A:1 D9_0 Male Chionoecetes bairdi 15.7 33 crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit Crandall
B:1 D9_1 Male Chionoecetes bairdi 17.6 33 crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit Crandall
C:1 D12_cold_0 Male Chionoecetes bairdi 24.9 33 crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit Crandall
D:1 D12_cold_1 Male Chionoecetes bairdi 26 33 crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit Crandall
E:1 D12_warm_0 Male Chionoecetes bairdi 26 33 crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit Crandall
F:1 D12_warm_1 Male Chionoecetes bairdi 24.4 33 crab hemolymph RNA TE Zymo Research: Quick-DNA/RNA Microprep Plus Kit Crandall

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