Kaitlyn’s notebook: Primers next steps/goals

  1. Look at product and compare to theoretical size
    • Must do visually (order based on sequence)
  2. Make plots for each primer w/ pooled
    • Cq.mean
    • Melt temp
    • Melt peak height
  3. Analyze Cq values
    • Sex or development differences (ANOVA?)
      • mean, melt temp and melt peak height by sex
        • And then by dev. stage
  4. Make summary of performance of each primer
    • Via a table (rank performances of each primer w/ grade & notes on grade [3 columns])
      • With pooled sample
      • And known samples