Kaitlyn’s notebook: Primers next steps/goals

1. Look at product and compare to theoretical size
   • Must do visually (order based on sequence)
2. Make plots for each primer w/ pooled
   • Cq.mean
   • Melt temp
   • Melt peak height
3. Analyze Cq values
   • Sex or development differences (ANOVA?)
     • mean, melt temp and melt peak height by sex
       • And then by dev. stage
4. Make summary of performance of each primer
   • Via a table (rank performances of each primer w/ grade & notes on grade [3 columns])
     • With pooled sample
     • And known samples