Sending samples for sequencing
Quotes obtained? Check. PO numbers generated? Check. Sequence submission forms submitted? Check.
Then there’s nothing left to do but send out the samples! I labelled screw top tubes for all samples with enough DNA and RNA yield based on the extraction metadata. Basically, this meant I labelled DNA and RNA tubes for every sample except for sample 57. I used screw top tubes because they’re more secure and I didn’t want any samples spilling! Once tubes were labelled, I grabbed the samples from the -80 ºC and let them equilibrate to room temperature. I centrifuged each sample to ensure all the liquid could easily be pipetted, and transferred it to the appropriate labelled screw top tube. I then put that tube in a labelled ziploc bag, using separate ones for DNA and RNA. I placed the filled ziploc bags with samples at the bottom of a cooler with an insulating lining. I then filled the lining with dry ice so there was ample ice to keep the samples cold. Once the samples were packaged properly, I sent the package to ZymoResearch via FedEx Overnight!
- Wait for sequencing data!