July 26 RNA Extraction

Today, I came into lab to work on RNA extraction with Sam. Sam had begun with the thawed homogenized RNA by the time I had entered. I pipetted the rest of the RNA into the column set up and then worked to finish the RNA extraction by following the protocol in the box. It went by smoothly. Due to the limited time we had, Sam took the lead in forming the cDNA. He guided me in understanding how cDNA is formed, and tomorrow, he will go in deeper description of how he did this process.

This is an image of the lining up of the tubes within the centrifuge
This is an image of the centrifuged tubes before DNAse was placed.