This morning begun with a lab meeting where we dived into the last chapter of Fresh Banana Leaves. The chapter focused on colonialism, eco-tourism and invasive species, among other topics. I will continue to work on the abstract today and tomorrow, I will come into lab to learn about using R studio with Professor Roberts.
Today, I came into lab to weigh the dried somatic and gonad tissues of the mussels 31-40. I placed a clean empty weigh boat and then tared it to zero before I weighed each dried tissue. Afterwards, I placed the tissues back into the oven and recorded the weights into the spreadsheet. Sam worked with me to get familiarized with using R by creating an account for me and walking me through parts of R studio. We played around with downloading the data into R, creating a graph and working with the labels.
Today, I begun this morning by working on the spreadsheet, and then I met with Professor Roberts to talk about the spreadsheet and to plan what will happen in the coming days. I asked questions about the RNA extraction process that I was unsure about, and what it could have meant to have no Cq values expressed. Professor Roberts showed me ways I need to consolidate information into one sheet – measurements of mussels (length, width and height), dry weight of the somatic and gonad tissues – along with the Cq mean values. This would allow us to find any relationship between any of the conditions. I was also introduced to the formula used to convert the Cq mean values into larger quantities that I can understand more easily, since the smaller the Cq, the more expression of the gene within the mussel. I will come in to weigh the dried out tissues tomorrow. We will also work on R next week Tuesday.