August 3rd Spreadsheet Work

Today, I begun this morning by working on the spreadsheet, and then I met with Professor Roberts to talk about the spreadsheet and to plan what will happen in the coming days. I asked questions about the RNA extraction process that I was unsure about, and what it could have meant to have no Cq values expressed. Professor Roberts showed me ways I need to consolidate information into one sheet – measurements of mussels (length, width and height), dry weight of the somatic and gonad tissues – along with the Cq mean values. This would allow us to find any relationship between any of the conditions. I was also introduced to the formula used to convert the Cq mean values into larger quantities that I can understand more easily, since the smaller the Cq, the more expression of the gene within the mussel. I will come in to weigh the dried out tissues tomorrow. We will also work on R next week Tuesday.