I’m done with my QuantSeq libraries! After getting a few quotes for sequencing, we’ve decided have the UW Genome Sciences sequencing core do it (the Sequencing Northwest Genomics Center). They also pooled my libraries for me for a small fee, but I needed to provide them with library concentrations (from QuBit). I also sent them my BioAnalyzer results (mean library length).
On Friday, April 17 I prepped my samples and walked them over to Erica at the genome science center. Here’s what needed to be done:
Quantified a few more libraries using Qubit.
I had to quantify my juvenile O. lurida libraries, and a couple random libraries that I previously omitted. I used the Qubit 1X dsDNA HS Assay.
Transerred libraries to new PCR plates
I submitted 8 uL of each library, which is ~half of my volumes (started with ~16 uL). I also wanted to make sure the sequencing facility would correctly pool my libraries in two batches – 1) Ctenidia+Juvenile and 2) Larvae. This is because some of my index numbers were the same in Batch 1 as in Batch 2, so they needed to be run on separate lanes. To make this very clear, I prepared 2 PCR plates, one for each batch/lane. Also, because I re-prepped a few libraries, I needed to carefully identify which to transfer, and which to omit.

Library details
Below are details for the libraries I submitted, with information such as original RNA concentration used for library prep, # PCR cycles used to amplify libraries, library concentration (DNA, from Qubit), mean library length (for a few), index number. All this information is located in this Sample-Submission-and-Info.xlsx spreadsheet.
Here is the manifest that I sent to Erica: Spencer,LH_Sample-Manifest-2020-04-17.xlsx
Here is the sample submission sheet: Spencer,LH_Sample-Submission-Form-2020-04-17.pdf
A note on PhiX Spike-in:
Erica asked whether I would like PhiX spike-in. Standard protocol for any Illumina platform is to do a 1% spike-in for quality control. The QuantSeq library prep manual does not specify whether it is recommended for my library type, although it does specify for QuantSeq libraries prepped using an add-on module which tack on UMIs to each library. I eailed my QuantSeq rep, and they replied quickly with the following: “For standard QuantSeq libraries without UMIs, 1% is fine. The reason libraries with UMIs need more is due to the spacer we use with the UMIs. It has a unique fingerprint that requires a more PhiX. That is the only reason. Even when labs combine non-UMI libraries with UMI libraries, they no longer need the higher percentage of PhiX. It is for UMI only libraries in a flow cell lane. It is rather specific.” So, in the end I had them use a 1% PhiX spike-in.
Adult O. lurida ctenidia samples, multiple populations and pCO2 exposures
Plate/Batch/Lane # |
Well |
Sample No. |
[RNA] (ng/ul) |
Cycles, round down (for most) |
Tissue source |
Tissue type |
[DNA] (ng/uL) |
Bioanalyzer mean bp |
INDEX # |
Index bp sequence |
Population |
Parental pCO2 |
---|
1 |
D06 |
291 |
158.0 |
15 |
Adult |
ctenidia |
3.54 |
NT |
7035 |
GTTACC |
Dabob Bay |
High |
1 |
C08 |
292 |
29.6 |
15 |
Adult |
ctenidia |
1.32 |
NT |
7037 |
TGGCGA |
Dabob Bay |
High |
1 |
A11 |
293 |
39.6 |
16 |
Adult |
ctenidia |
1.03 |
NT |
7014 |
AATCCG |
Dabob Bay |
High |
1 |
A07 |
294 |
110.0 |
16 |
Adult |
ctenidia |
1.95 |
245 |
7008 |
TGTGCA |
Dabob Bay |
High |
1 |
D05 |
295 |
34.8 |
15 |
Adult |
ctenidia |
3.58 |
NT |
7050 |
TCGAGG |
Dabob Bay |
High |
1 |
D04 |
296 |
180.0 |
15 |
Adult |
ctenidia |
3.46 |
NT |
7046 |
CTCCAT |
Dabob Bay |
High |
1 |
C01 |
298 |
182.0 |
15 |
Adult |
ctenidia |
3.08 |
NT |
7028 |
GCTCGA |
Dabob Bay |
High |
1 |
B09 |
299 |
50.4 |
15 |
Adult |
ctenidia |
2.46 |
NT |
7024 |
CCGCAA |
Dabob Bay |
High |
1 |
B10 |
301 |
75.8 |
15 |
Adult |
ctenidia |
2.82 |
NT |
7025 |
TTTATG |
Dabob Bay |
Ambient |
1 |
B03 |
302 |
62.4 |
14 |
Adult |
ctenidia |
4.9 |
NT |
7018 |
GTCAGG |
Dabob Bay |
Ambient |
1 |
B05 |
303 |
95.2 |
14 |
Adult |
ctenidia |
2.4 |
NT |
7020 |
TATGTC |
Dabob Bay |
Ambient |
1 |
B12 |
304 |
200.0 |
15 |
Adult |
ctenidia |
2.8 |
NT |
7027 |
CAAGCA |
Dabob Bay |
Ambient |
1 |
A01 |
305 |
75.2 |
16 |
Adult |
ctenidia |
2.96 |
267 |
7002 |
GATCAC |
Dabob Bay |
Ambient |
1 |
D08 |
306 |
136.0 |
15 |
Adult |
ctenidia |
4.14 |
NT |
7051 |
CACTAA |
Dabob Bay |
Ambient |
1 |
D09 |
307 |
89.4 |
15 |
Adult |
ctenidia |
3.32 |
NT |
7053 |
CGCCTG |
Dabob Bay |
Ambient |
1 |
A05 |
308 |
73.6 |
16 |
Adult |
ctenidia |
2.48 |
256 |
7006 |
GTGTAG |
Dabob Bay |
Ambient |
1 |
B08 |
309 |
170.0 |
14 |
Adult |
ctenidia |
1.46 |
NT |
7023 |
CACACT |
Dabob Bay |
Ambient |
1 |
A02 |
311 |
158.0 |
16 |
Adult |
ctenidia |
3.76 |
262 |
7003 |
ACCAGT |
Oyster Bay C1 |
High |
1 |
C04 |
312 |
90.6 |
15 |
Adult |
ctenidia |
1.58 |
NT |
7032 |
CGAAGG |
Oyster Bay C1 |
High |
1 |
C12 |
313 |
72.4 |
15 |
Adult |
ctenidia |
1.81 |
NT |
7041 |
CTCTCG |
Oyster Bay C1 |
High |
1 |
E01 |
314 |
42.2 |
17 |
Adult |
ctenidia |
4.32 |
NT |
7049 |
GTGCCA |
Oyster Bay C1 |
High |
1 |
C02 |
315 |
148.0 |
15 |
Adult |
ctenidia |
2.32 |
NT |
7029 |
GCGAAT |
Oyster Bay C1 |
High |
1 |
A09 |
316 |
146.0 |
16 |
Adult |
ctenidia |
3.74 |
252 |
7012 |
ATGAAC |
Oyster Bay C1 |
High |
1 |
C09 |
317 |
158.0 |
15 |
Adult |
ctenidia |
2.14 |
NT |
7038 |
ACCGTG |
Oyster Bay C1 |
High |
1 |
A06 |
318 |
174.0 |
16 |
Adult |
ctenidia |
4.08 |
262 |
7007 |
CTAGTC |
Oyster Bay C1 |
High |
1 |
E04 |
319 |
77.6 |
17 |
Adult |
ctenidia |
3.02 |
NT |
7011 |
TTAACT |
Oyster Bay C1 |
High |
1 |
C05 |
321 |
148.0 |
15 |
Adult |
ctenidia |
3.64 |
NT |
7033 |
AGATAG |
Oyster Bay C1 |
Ambient |
1 |
A08 |
322 |
44.6 |
16 |
Adult |
ctenidia |
2.7 |
269 |
7010 |
CGGTTA |
Oyster Bay C1 |
Ambient |
1 |
D03 |
323 |
102.0 |
15 |
Adult |
ctenidia |
3.7 |
NT |
7044 |
ACAGAT |
Oyster Bay C1 |
Ambient |
1 |
D11 |
324 |
172.0 |
16 |
Adult |
ctenidia |
2.84 |
NT |
7009 |
TCAGGA |
Oyster Bay C1 |
Ambient |
1 |
B01 |
325 |
180.0 |
14 |
Adult |
ctenidia |
2.92 |
NT |
7016 |
TACCTT |
Oyster Bay C1 |
Ambient |
1 |
D02 |
326 |
130.0 |
15 |
Adult |
ctenidia |
4.04 |
NT |
7043 |
AAGACA |
Oyster Bay C1 |
Ambient |
1 |
D01 |
327 |
85.2 |
15 |
Adult |
ctenidia |
1.97 |
NT |
7042 |
TGACAC |
Oyster Bay C1 |
Ambient |
1 |
A12 |
328 |
156.0 |
14 |
Adult |
ctenidia |
2.12 |
NT |
7015 |
GGCTGC |
Oyster Bay C1 |
Ambient |
1 |
D10 |
329 |
162.0 |
16 |
Adult |
ctenidia |
5.1 |
NT |
7045 |
TAGGCT |
Oyster Bay C1 |
Ambient |
1 |
E05 |
331 |
42.2 |
19 |
Adult |
ctenidia |
9.6 |
NT |
7001 |
CAGCGT |
Fidalgo Bay |
High |
1 |
D12 |
332 |
65.8 |
16 |
Adult |
ctenidia |
2.14 |
NT |
7047 |
GCATGG |
Fidalgo Bay |
High |
1 |
C06 |
333 |
78.6 |
15 |
Adult |
ctenidia |
2.98 |
NT |
7034 |
TTGGTA |
Fidalgo Bay |
High |
1 |
E02 |
334 |
64.8 |
17 |
Adult |
ctenidia |
3.92 |
NT |
7048 |
AATAGC |
Fidalgo Bay |
High |
1 |
C07 |
335 |
180.0 |
15 |
Adult |
ctenidia |
2.58 |
NT |
7036 |
CGCAAC |
Fidalgo Bay |
High |
1 |
E03 |
336 |
94.8 |
17 |
Adult |
ctenidia |
4.6 |
NT |
7052 |
GGTATA |
Fidalgo Bay |
High |
1 |
C11 |
337 |
194.0 |
15 |
Adult |
ctenidia |
4.02 |
NT |
7040 |
GATTGT |
Fidalgo Bay |
High |
1 |
A04 |
338 |
81.6 |
16 |
Adult |
ctenidia |
2.74 |
269 |
7005 |
ACATTA |
Fidalgo Bay |
High |
1 |
A10 |
339 |
77.2 |
16 |
Adult |
ctenidia |
1.91 |
NT |
7013 |
CCTAAG |
Fidalgo Bay |
High |
1 |
B07 |
341 |
89.6 |
14 |
Adult |
ctenidia |
1.31 |
NT |
7022 |
GGAGGT |
Fidalgo Bay |
Ambient |
1 |
B11 |
342 |
162.0 |
15 |
Adult |
ctenidia |
1.78 |
NT |
7026 |
AACGCC |
Fidalgo Bay |
Ambient |
1 |
B02 |
343 |
114.0 |
14 |
Adult |
ctenidia |
2.58 |
NT |
7017 |
TCTTAA |
Fidalgo Bay |
Ambient |
1 |
C03 |
344 |
25.0 |
15 |
Adult |
ctenidia |
1.58 |
NT |
7030 |
TGGATT |
Fidalgo Bay |
Ambient |
1 |
B04 |
345 |
190.0 |
14 |
Adult |
ctenidia |
3.5 |
NT |
7019 |
ATACTG |
Fidalgo Bay |
Ambient |
1 |
B06 |
346 |
43.6 |
14 |
Adult |
ctenidia |
1.36 |
NT |
7021 |
GAGTCC |
Fidalgo Bay |
Ambient |
1 |
D07 |
347 |
69.0 |
15 |
Adult |
ctenidia |
4.36 |
NT |
7031 |
ACCTAC |
Fidalgo Bay |
Ambient |
1 |
A03 |
348 |
54.4 |
16 |
Adult |
ctenidia |
3.06 |
265 |
7004 |
TGCACG |
Fidalgo Bay |
Ambient |
1 |
C10 |
349 |
82.0 |
15 |
Adult |
ctenidia |
1.9 |
NT |
7039 |
CAACAG |
Fidalgo Bay |
Ambient |
Juvenile O. lurida whole-body samples from those deployed in Port Gamble, with two populations and two parental pCO2 exposures
Plate/Batch/Lane # |
Well |
Sample No. |
[RNA] (ng/ul) |
Cycles, round down (for most) |
Tissue source |
Tissue type |
[DNA] (ng/uL) |
Bioanalyzer mean bp |
INDEX # |
Index bp sequence |
Population |
Parental pCO2 |
---|
1 |
G02 |
137 |
99.2 |
15 |
Juvenile |
whole body, individual |
5.2 |
NT |
7090 |
CCTGCT |
Dabob Bay |
Ambient |
1 |
F01 |
139 |
136.0 |
14 |
Juvenile |
whole body, individual |
13.3 |
294 |
7081 |
GCAGCC |
Dabob Bay |
Ambient |
1 |
G03 |
140 |
174.0 |
15 |
Juvenile |
whole body, individual |
7.62 |
314 |
7091 |
GCGCTG |
Dabob Bay |
Ambient |
1 |
F08 |
141 |
190.0 |
15 |
Juvenile |
whole body, individual |
23.6 |
NT |
7088 |
AGACCA |
Dabob Bay |
Ambient |
1 |
G05 |
156 |
110.0 |
16 |
Juvenile |
whole body, individual |
4.32 |
NT |
7093 |
TTCGAG |
Fidalgo Bay |
Ambient |
1 |
G07 |
159 |
184.0 |
16 |
Juvenile |
whole body, individual |
23 |
283 |
7095 |
AGGCAT |
Fidalgo Bay |
Ambient |
1 |
F04 |
161 |
168.0 |
14 |
Juvenile |
whole body, individual |
6.22 |
290 |
7084 |
AAGTGG |
Fidalgo Bay |
Ambient |
1 |
G01 |
162 |
164 |
15 |
Juvenile |
whole body, individual |
6.74 |
289 |
7080 |
AACAAG |
Fidalgo Bay |
Ambient |
1 |
G06 |
168 |
89.0 |
16 |
Juvenile |
whole body, individual |
15.9 |
305 |
7094 |
AGAATC |
Dabob Bay |
High |
1 |
G04 |
169 |
95.6 |
16 |
Juvenile |
whole body, individual |
3.68 |
310 |
7092 |
GAACCT |
Dabob Bay |
High |
1 |
F05 |
171 |
146.0 |
15 |
Juvenile |
whole body, individual |
3.22 |
305 |
7085 |
CTCATA |
Dabob Bay |
High |
1 |
F06 |
172 |
116.0 |
15 |
Juvenile |
whole body, individual |
10.6 |
NT |
7086 |
CCGACC |
Dabob Bay |
High |
1 |
G08 |
181 |
138.0 |
16 |
Juvenile |
whole body, individual |
2.84 |
280 |
7096 |
ACACGC |
Fidalgo Bay |
High |
1 |
F03 |
183 |
196.0 |
14 |
Juvenile |
whole body, individual |
5.88 |
NT |
7083 |
TGCTAT |
Fidalgo Bay |
High |
1 |
F02 |
184 |
96.4 |
14 |
Juvenile |
whole body, individual |
6.1 |
NT |
7082 |
ACTCTT |
Fidalgo Bay |
High |
1 |
F07 |
185 |
102.0 |
15 |
Juvenile |
whole body, individual |
15 |
296 |
7087 |
GGCCAA |
Fidalgo Bay |
High |
Larval O. lurida, multiple cohorts, parental pCO2 and temperature exposures. Larvae are pooled by pulse/family.
Plate/Batch/Lane # |
Well |
Sample No. |
[RNA] (ng/ul) |
Vol RNA used |
Cycles, round down (for most) |
Tissue source |
Tissue type |
[DNA] (ng/uL) |
Bioanalyzer mean bp |
INDEX # |
Index bp sequence |
Population |
Parental Temperature |
Parental pCO2 |
Larval sample # |
---|
2 |
F08 |
34 |
104.0 |
3.37 |
19 |
Larval |
whole body, pooled |
3.8 |
NT |
7073 |
GACATC |
Oyster Bay C1 |
6 |
Ambient |
77-A |
2 |
F12 |
35 |
94.4 |
3.71 |
21 |
Larval |
whole body, pooled |
0.522 |
NT |
7075 |
CGTCGC |
Oyster Bay C1 |
6 |
Ambient |
10-A |
2 |
F10 |
37 |
74.6 |
4.69 |
21 |
Larval |
whole body, pooled |
5.46 |
NT |
7076 |
ATGGCG |
Oyster Bay C1 |
6 |
Ambient |
69-A |
2 |
E04 |
39 |
89.8 |
3.90 |
15 |
Larval |
whole body, pooled |
2.04 |
NT |
7056 |
ATATCC |
Oyster Bay C1 |
6 |
Ambient |
48-A |
2 |
F02 |
41 |
108.0 |
3.24 |
19 |
Larval |
whole body, pooled |
6.82 |
NT |
7068 |
CCAATT |
Oyster Bay C1 |
10 |
High |
06-A |
2 |
D10 |
43 |
156.0 |
2.24 |
15 |
Larval |
whole body, pooled |
2.48 |
261 |
7050 |
TCGAGG |
Oyster Bay C1 |
10 |
High |
08-A |
2 |
F11 |
44 |
41.4 |
5.00 |
21 |
Larval |
whole body, pooled |
0.18 |
NT |
7078 |
GCCACA |
Oyster Bay C1 |
10 |
High |
32-A |
2 |
F03 |
45 |
25.1 |
5 |
19 |
Larval |
whole body, pooled |
4.8 |
NT |
7077 |
ATTGGT |
Oyster Bay C1 |
10 |
High |
79-A |
2 |
F06 |
46 |
98.6 |
3.55 |
19 |
Larval |
whole body, pooled |
4.52 |
NT |
7074 |
CGATCT |
Oyster Bay C1 |
10 |
High |
24-A |
2 |
E10 |
47 |
128.0 |
2.73 |
16 |
Larval |
whole body, pooled |
2.58 |
NT |
7070 |
AACCGA |
Oyster Bay C1 |
10 |
High |
26-A |
2 |
A10 |
401 |
93.4 |
3.75 |
16 |
Larval |
whole body, pooled |
1.24 |
325 |
7012 |
ATGAAC |
Dabob Bay |
10 |
Ambient |
14-A |
2 |
A05 |
402 |
114.0 |
3.07 |
16 |
Larval |
whole body, pooled |
2.76 |
346 |
7006 |
GTGTAG |
Dabob Bay |
10 |
Ambient |
31-A |
2 |
C06 |
403 |
136.0 |
2.57 |
14 |
Larval |
whole body, pooled |
4.16 |
NT |
7032 |
CGAAGG |
Dabob Bay |
10 |
Ambient |
75-A |
2 |
D06 |
404 |
112.0 |
3.13 |
15 |
Larval |
whole body, pooled |
2.92 |
NT |
7046 |
CTCCAT |
Dabob Bay |
10 |
Ambient |
80-A |
2 |
A09 |
411 |
72.6 |
4.82 |
16 |
Larval |
whole body, pooled |
3.08 |
288 |
7011 |
TTAACT |
Dabob Bay |
10 |
High |
23-A |
2 |
G02 |
412 |
31.2 |
5.00 |
23 |
Larval |
whole body, pooled |
2.52 |
NT |
7069 |
AGTTGA |
Dabob Bay |
10 |
High |
27-A |
2 |
D02 |
413 |
130.0 |
2.69 |
15 |
Larval |
whole body, pooled |
1.97 |
NT |
7041 |
CTCTCG |
Dabob Bay |
10 |
High |
58-A |
2 |
E01 |
414 |
168.0 |
2.08 |
15 |
Larval |
whole body, pooled |
0.858 |
NT |
7053 |
CGCCTG |
Dabob Bay |
10 |
High |
60-A |
2 |
B10 |
421 |
57.6 |
5.00 |
14 |
Larval |
whole body, pooled |
1.63 |
NT |
7024 |
CCGCAA |
Dabob Bay |
6 |
Ambient |
59-A |
2 |
G03 |
432 |
74.0 |
4.73 |
23 |
Larval |
whole body, pooled |
6.28 |
NT |
7065 |
AAGCTC |
Dabob Bay |
6 |
High |
72-A |
2 |
E07 |
434 |
162.0 |
2.16 |
15 |
Larval |
whole body, pooled |
5.58 |
NT |
7059 |
GGTGAG |
Dabob Bay |
6 |
High |
74-A |
2 |
G01 |
441 |
16.2 |
5.00 |
23 |
Larval |
whole body, pooled |
0.866 |
NT |
7061 |
GAAGTG |
Fidalgo Bay |
10 |
Ambient |
20-A |
2 |
C12 |
443 |
60.2 |
5.00 |
15 |
Larval |
whole body, pooled |
1.36 |
NT |
7039 |
CAACAG |
Fidalgo Bay |
10 |
Ambient |
53-A |
2 |
C10 |
444 |
70.6 |
4.96 |
14 |
Larval |
whole body, pooled |
4.08 |
NT |
7037 |
TGGCGA |
Fidalgo Bay |
10 |
Ambient |
63-A |
2 |
D09 |
445 |
160.0 |
2.19 |
15 |
Larval |
whole body, pooled |
4 |
NT |
7049 |
GTGCCA |
Fidalgo Bay |
10 |
Ambient |
65-A |
2 |
C04 |
451 |
68.4 |
5.12 |
14 |
Larval |
whole body, pooled |
2.24 |
NT |
7030 |
TGGATT |
Fidalgo Bay |
10 |
High |
16-A |
2 |
A12 |
453 |
196.0 |
1.79 |
14 |
Larval |
whole body, pooled |
0.968 |
NT |
7014 |
AATCCG |
Fidalgo Bay |
10 |
High |
36-A |
2 |
B08 |
473 |
124.0 |
2.82 |
14 |
Larval |
whole body, pooled |
2.34 |
NT |
7022 |
GGAGGT |
Fidalgo Bay |
6 |
High |
46-A |
2 |
B02 |
474 |
77.2 |
4.53 |
14 |
Larval |
whole body, pooled |
3.02 |
NT |
7016 |
TACCTT |
Fidalgo Bay |
6 |
High |
47-A |
2 |
B04 |
475 |
27.4 |
5.00 |
14 |
Larval |
whole body, pooled |
2.44 |
NT |
7018 |
GTCAGG |
Fidalgo Bay |
6 |
High |
50-A |
2 |
B05 |
476 |
39.2 |
5.00 |
14 |
Larval |
whole body, pooled |
4.24 |
NT |
7019 |
ATACTG |
Fidalgo Bay |
6 |
High |
54-A |
2 |
D01 |
477 |
164.0 |
2.13 |
15 |
Larval |
whole body, pooled |
2.28 |
NT |
7040 |
GATTGT |
Fidalgo Bay |
6 |
High |
76-A |
2 |
E09 |
481 |
89.2 |
3.92 |
15 |
Larval |
whole body, pooled |
2.18 |
NT |
7036 |
CGCAAC |
Oyster Bay C1 |
10 |
Ambient |
02-A |
2 |
C01 |
482 |
22.2 |
5.00 |
14 |
Larval |
whole body, pooled |
1.42 |
NT |
7027 |
CAAGCA |
Oyster Bay C1 |
10 |
Ambient |
04-A |
2 |
A07 |
483 |
99.0 |
3.54 |
16 |
Larval |
whole body, pooled |
1.22 |
219 |
7009 |
TCAGGA |
Oyster Bay C1 |
10 |
Ambient |
03-A |
2 |
D07 |
484 |
58.4 |
5.00 |
15 |
Larval |
whole body, pooled |
1.67 |
NT |
7047 |
GCATGG |
Oyster Bay C1 |
10 |
Ambient |
09-A |
2 |
B09 |
485 |
19.1 |
5.00 |
14 |
Larval |
whole body, pooled |
2.9 |
NT |
7023 |
CACACT |
Oyster Bay C1 |
10 |
Ambient |
34-A |
2 |
A06 |
487 |
118.0 |
2.97 |
16 |
Larval |
whole body, pooled |
1.27 |
290 |
7007 |
CTAGTC |
Oyster Bay C1 |
10 |
Ambient |
40-A |
2 |
C02 |
488 |
60.0 |
5.00 |
14 |
Larval |
whole body, pooled |
3.06 |
NT |
7028 |
GCTCGA |
Oyster Bay C1 |
10 |
Ambient |
44-A |
2 |
C11 |
489 |
68.0 |
5.00 |
15 |
Larval |
whole body, pooled |
3.84 |
272 |
7038 |
ACCGTG |
Oyster Bay C1 |
10 |
Ambient |
49-A |
2 |
B07 |
490 |
186.0 |
1.88 |
14 |
Larval |
whole body, pooled |
2.32 |
NT |
7021 |
GAGTCC |
Oyster Bay C1 |
10 |
Ambient |
64-A |
2 |
E02 |
491 |
58.4 |
5.00 |
15 |
Larval |
whole body, pooled |
6.64 |
NT |
7054 |
AATGAA |
Oyster Bay C1 |
10 |
Ambient |
66-A |
2 |
D04 |
492 |
82.6 |
4.24 |
15 |
Larval |
whole body, pooled |
2.02 |
NT |
7043 |
AAGACA |
Oyster Bay C1 |
10 |
Ambient |
81-A |
2 |
D11 |
506 |
29.2 |
5.00 |
15 |
Larval |
whole body, pooled |
1.63 |
NT |
7051 |
CACTAA |
Oyster Bay C1 |
10 |
High |
62-A |
2 |
E08 |
513 |
142.0 |
2.46 |
15 |
Larval |
whole body, pooled |
5.92 |
NT |
7060 |
TTCCGC |
Oyster Bay C1 |
6 |
Ambient |
45-A |
2 |
F05 |
521 |
66.6 |
5.00 |
19 |
Larval |
whole body, pooled |
8.66 |
NT |
7071 |
CAGATG |
Oyster Bay C1 |
6 |
High |
01-A |
2 |
A01 |
522 |
32.2 |
5.00 |
16 |
Larval |
whole body, pooled |
2.42 |
NT |
7002 |
GATCAC |
Oyster Bay C1 |
6 |
High |
07-A |
2 |
A04 |
523 |
71.4 |
4.90 |
16 |
Larval |
whole body, pooled |
2.16 |
276 |
7005 |
ACATTA |
Oyster Bay C1 |
6 |
High |
25-A |
2 |
A11 |
524 |
63.0 |
5.00 |
14 |
Larval |
whole body, pooled |
3.24 |
NT |
7013 |
CCTAAG |
Oyster Bay C1 |
6 |
High |
28-A |
2 |
B06 |
525 |
140.0 |
2.50 |
14 |
Larval |
whole body, pooled |
2.44 |
NT |
7020 |
TATGTC |
Oyster Bay C1 |
6 |
High |
30-A |
2 |
B03 |
526 |
138.0 |
2.54 |
14 |
Larval |
whole body, pooled |
2.2 |
NT |
7017 |
TCTTAA |
Oyster Bay C1 |
6 |
High |
33-A |
2 |
D03 |
527 |
124.0 |
2.82 |
15 |
Larval |
whole body, pooled |
1.42 |
243 |
7042 |
TGACAC |
Oyster Bay C1 |
6 |
High |
61-A |
2 |
C03 |
528 |
87.6 |
4.00 |
14 |
Larval |
whole body, pooled |
4.48 |
NT |
7029 |
GCGAAT |
Oyster Bay C1 |
6 |
High |
68-A |
2 |
E05 |
529 |
56.8 |
5.00 |
15 |
Larval |
whole body, pooled |
2.86 |
NT |
7057 |
AGTACT |
Oyster Bay C1 |
6 |
High |
70-A |
2 |
D08 |
531 |
95.4 |
3.67 |
15 |
Larval |
whole body, pooled |
2.1 |
NT |
7048 |
AATAGC |
Oyster Bay C2 |
10 |
Ambient |
17-A |
2 |
C09 |
532 |
93.6 |
3.74 |
14 |
Larval |
whole body, pooled |
2.12 |
NT |
7035 |
GTTACC |
Oyster Bay C2 |
10 |
Ambient |
42-A |
2 |
F01 |
533 |
6.5 |
5.00 |
17 |
Larval |
whole body, pooled |
5.36 |
NT |
7063 |
ACGTCT |
Oyster Bay C2 |
10 |
Ambient |
56-A |
2 |
D05 |
541 |
44.4 |
5.00 |
15 |
Larval |
whole body, pooled |
1.4 |
NT |
7044 |
ACAGAT |
Oyster Bay C2 |
10 |
High |
12-A |
2 |
A03 |
542 |
32.8 |
5.00 |
16 |
Larval |
whole body, pooled |
2.22 |
270 |
7004 |
TGCACG |
Oyster Bay C2 |
10 |
High |
13-A |
2 |
C05 |
543 |
162.0 |
2.16 |
14 |
Larval |
whole body, pooled |
5.24 |
NT |
7031 |
ACCTAC |
Oyster Bay C2 |
10 |
High |
43-A |
2 |
B12 |
551 |
96.4 |
3.63 |
14 |
Larval |
whole body, pooled |
1.69 |
NT |
7026 |
AACGCC |
Oyster Bay C2 |
6 |
Ambient |
35-A |
2 |
B11 |
553 |
186.0 |
1.88 |
14 |
Larval |
whole body, pooled |
3.28 |
NT |
7025 |
TTTATG |
Oyster Bay C2 |
6 |
Ambient |
55-A |
2 |
B01 |
554 |
188.0 |
1.86 |
14 |
Larval |
whole body, pooled |
2.38 |
NT |
7015 |
GGCTGC |
Oyster Bay C2 |
6 |
Ambient |
78-A |
2 |
F09 |
561 |
28.0 |
5.00 |
21 |
Larval |
whole body, pooled |
4.1 |
NT |
7066 |
GACGAT |
Oyster Bay C2 |
6 |
High |
05-A |
2 |
G05 |
562 |
126 |
2.777778 |
23 |
Larval |
whole body, pooled |
9.88 |
NT |
7064 |
CAGGAC |
Oyster Bay C2 |
6 |
High |
11-A |
2 |
E11 |
563 |
47.0 |
5.00 |
16 |
Larval |
whole body, pooled |
4.66 |
NT |
7045 |
TAGGCT |
Oyster Bay C2 |
6 |
High |
15-A |
2 |
C08 |
564 |
152.0 |
2.30 |
14 |
Larval |
whole body, pooled |
2.24 |
NT |
7034 |
TTGGTA |
Oyster Bay C2 |
6 |
High |
52-A |
2 |
F07 |
565 |
31.2 |
5.00 |
19 |
Larval |
whole body, pooled |
3.2 |
NT |
7067 |
TCGTTC |
Oyster Bay C2 |
6 |
High |
57-A |
2 |
G04 |
571 |
LOW |
5.00 |
23 |
Larval |
whole body, pooled |
0.232 |
287 |
7072 |
GTAGAA |
Negative control |
NA |
NA |
NA |
2 |
A08 |
431b |
83.0 |
4.22 |
16 |
Larval |
whole body, pooled |
3.36 |
285 |
7010 |
CGGTTA |
Dabob Bay |
6 |
High |
51-A |
2 |
E12 |
442b |
69.8 |
5.00 |
17 |
Larval |
whole body, pooled |
3.2 |
NT |
7062 |
CAATGC |
Fidalgo Bay |
10 |
Ambient |
38-A |
2 |
A02 |
452b |
97.2 |
3.60 |
16 |
Larval |
whole body, pooled |
2.68 |
294 |
7003 |
ACCAGT |
Fidalgo Bay |
10 |
High |
18-A |
2 |
C07 |
461b |
84.0 |
4.17 |
14 |
Larval |
whole body, pooled |
2.02 |
NT |
7033 |
AGATAG |
Fidalgo Bay |
6 |
Ambient |
22-A |
2 |
F04 |
462b |
106 |
3.301887 |
19 |
Larval |
whole body, pooled |
8.46 |
NT |
7001 |
CAGCGT |
Fidalgo Bay |
6 |
Ambient |
29-A |
2 |
E03 |
471b |
108.0 |
3.24 |
15 |
Larval |
whole body, pooled |
5.2 |
NT |
7055 |
ACAACG |
Fidalgo Bay |
6 |
High |
19-A |
2 |
D12 |
472b |
97.0 |
3.61 |
15 |
Larval |
whole body, pooled |
2.42 |
NT |
7052 |
GGTATA |
Fidalgo Bay |
6 |
High |
21-A |
2 |
E06 |
552b |
74.6 |
4.69 |
15 |
Larval |
whole body, pooled |
1.84 |
NT |
7058 |
ATAAGA |
Oyster Bay C2 |
6 |
Ambient |
41-A |
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