Procedure & Results for Resazurin with Stressed Oysters

Procedure- 

1. Prepared a 0.1 M solution of resazurin by making 9.890 mL of DI water, 0.100 mL of pure resazurin, and 0.010 mL of DMSO.
2. 3 out of 4 columns of a sample tray were then filled with 2 mL of this solution, and one column would contain stressed oysters, one would contain non-stressed oysters, and the last column would be a control.
3.  Then, small oysters were then added to their respective columns, and then a spectrophotometer was used to track the absorbance over time.
4. In the spectrophotometer, measurements were taken every 15 minutes for 2 hours, and the lid was removed during measurements.

Results-

	Time (h)	0	0.25	0.5	0.75	1	1.25	1.5	1.75	2
CO1	A01	8315	–	8080	8327	8822	8549	8320	8594	8443
SO1	A02	8223	–	7267	7225	7134	7208	7106	7157	7792
Control1	A03	8355	–	7237	7082	7329	7120	7204	7148	7059
Blank	A04	8691	–	8568	8487	8580	8528	8489	8559	8399
CO2	B01	8499	–	8014	8094	7947	8530	8471	8738	8614
SO2	B02	8661	–	8579	8316	8769	8299	8019	8110	8732
Control2	B03	8695	–	7428	7244	7083	7294	7474	7345	7459
Blank	B04	8683	–	8376	8477	8660	8532	8552	8653	8407
CO3	C01	8302	–	8404	8700	8769	8477	8384	7772	8314
SO3	C02	8535	–	8840	8432	8202	7915	8440	8877	8107
Control3	C03	8500	–	7686	7527	7446	7428	7693	7520	7839
Blank	C04	8588	–	8371	8362	8383	8355	8542	8579	8453

SO- Stressed Oyster, CO- Control Oyster

Error occurred in the reading at 0.25 hours, so there is no data for this time.

Analysis- 

There was no noticeable change in the color of the wells, and the intensity remained relatively constant across all of the wells. This is could be due to the use of DI water in the solution instead of salt water.