Histology: Finished up the histology slide images of Oly and gigas gonad tissue. I uploaded the images into folders in the GitHub repository here. Additionally, I sent off the next batch of histology cassettes to be made into histology slides.
Oysters: Fed the oysters in the tank algae paste. Let sit for 1 hour. Measured salinity: 30. Started a feed log in order to keep track of salinity and feeding schedule.
I took pictures of the tissues of a few histology slides from Laura’s O. lurida project.
Below are some thoughts I have on what we may be looking at using my past experience of looking at geoduck gonad histology, and using the histological work from two papers (Oates 2013 thesis) (Moore et al., 2016) found from a recent literature search on specifically O. lurida gonadal histology. Also, Brent came over and helped a lot. (Link to google drive folder)
Ones I’m unsure about:
Histology from Moore et al. 2016:
Set up an iPod camera on a microscope for nearing histology work:
There is a glare, so either will have to be blocked by hand, or turn off overhead lights. Here is a google drive folder with some histology images.
Finished up some summary data for Rhonda’s oyster seed morphology:
Looked into some options for cell phone adapters for microscopes. Found a promising one from Gosky Universal.
Revisited edits to the “Differential response to stress in Ostrea lurida as measured by gene expression” paper. More needs to be done.
Started cleaning/organizing refrigerator in FTR 209. Added general shelf contents to the lab inventory spreadsheet under the “Refrigerator Rm 209” tab.
Information from Dr. Abraham Aviv, Rutgers
Samples were provided by SR Lab
First analysis phase: DNA integrity
9 samples degraded: loss of margins of the dense DNA band (crown) and presence of high intensity tail (smear)
Rest are ok.
Up next: measuring terminal restriction fragment length (telomere length: TL) in the good samples
Issue: whether or not TL will be within range to use Southern blot methodology
Yesterday morning Yaamini and I met Olivia at 8am at Manchester. We cleaned algae lines and filters. We performed water chemistry. Next week Yaamini will join us again, but will be mostly there to observe how well Olivia and I can perform the tasks, as we will do them ourselves while Yaamini and Laura are away.
Yesterday I sent out histology samples of gigas and olys from Laura and Yaamini’s projects. For future reference, this is what the histology filled-out form looks like that accompanies the samples.
Today I have continued Rhonda’s oyster seed measurements. I am currently beginning Tube 3, screen size 710-1000.