Grace’s Notebook: June 23, 2017

Today I scrounged around for missing data from Heare et. al paper (Evidence of Ostrea lurida Carpenter, 1864 population structure in Puget Sound, WA).

Found it!

Am now going through all the revisions and making sure that they were addressed. Sending the final version to Brent and Steven today, and will resubmit by Monday!



Grace’s Notebook: June 21, 2017

  1. Today I took pictures of all (except Silo 9 Day 13) oyster seed cryotubes so that I can more easily measure them using ImageJ whenever I have free time.
  2. I also did some scrounging around for missing data for revisions to the Heare et. al paper – Evidence of Ostrea lurida (Carpenter 1864) population structure in Puget Sound, WA. Found some of it in a master data sheet (link: here) and am still looking for some survival data. Link to Jake’s online notebook blog kept during the project: here.
  3. I also took some images of some larvae that are in 50ml tubes. The goal is to image them such that one image has 100 easily-countable larvae. It looks like the tubes have a  lot of things in there that aren’t larvae. Here are the images:

    Will work on this more Friday when I’m in.

Tomorrow (Thursday) I’m off to Manchester to assist Laura!

Grace’s Notebook: June 19, 2017 Manchester

Helped Laura today at Manchester along with Olivia.

I screened the larvae at 224, 180, and 100. Laura counted the larvae. Olivia brought the buckets over, switched out droppers and air stones, and cleaned.

After all that, I screened the new larvae from the oysters that spawned on 100 micron screens. These were placed in tri-pours and counted. Then, it was determined how much of the water containing the larvae should be re-stocked in the large buckets in order to stock 50K larvae. The remaining larvae were preserved in ethanol.

Grace’s Notebook: June 14, 2017

(1) Oyster seed morphology:

Continuing with measuring the lengths of oyster seed for Rhonda’s project. I think I will have to re-do Silo 3 and 9 from 7/14/16 because I didn’t get 100 seed pictured. Link to spreadsheet here. Link to images here.

(2) Minor revisions on Jake’s paper: Evidence of Ostrea lurida (Carpenter, 1864) population structure in Puget Sound, WA (link to PeerJ Preprint: here.)

Most of the revisions were references and typos. Other revision comments were sent to Steven to be changed for the final resubmission (Due by June 26th).

Grace’s Notebook: June 12, 2017

Day at Manchester:

Helped Laura with her project by screening for oyster larvae! I really enjoyed this task. I screened the larvae at 224, 180, and 100 microns. I then screened a mortality bucket at 100 microns. Will go out again this Thursday.

Grace’s Notebook: June 9, 2017

Today I helped Yaamini with desalting her oyster samples.

The purpose of the process is to break the proteins into peptides because peptides are more stable (won’t degrade). Here’s a link to her notebook entry which details the process: here.

I also continued the oyster seed morphology measurements for Rhonda – nearing the end!

Grace’s Notebook: June 6, 2017

More oyster seed morphology for Rhonda: link to spreadsheet: here; link to images folder: here.