Ronit’s Notebook: DNMT1 and Actin qPCR

In the last two qPCR assays, no fluorescence was detected. Turns out we were using Promega master mix which is specifically for probe-based assays…as such, it doesn’t have any fluorescent dye in it, explaining the lack of fluorescence detection in any of the assays. I switched over to using a different master mix that should solve the issue.

I reran the DNMT1 primer assays and will run an actin qPCR to determine if it might be a possible normalizing gene. For protocol used, please refer to previous lab book entries.

Link to Excel spreadsheet of Cq values for both actin and DNMT1:

Link to DNMT1 qPCR data:

Link to actin qPCR data:

Plate map:
All replicates are in adjacent wells. The order of the samples in the well plate by rows is: D01, D02, D03, D04, D05, D06, D07, D08, D09, D10, D11, D12, D13, D14, D15, D16, D17, D18, D19, D20, T01, T02, T03, T04, T05, T06, T07, T08, T09, T10, T11, T12, T13, T14, T15, T16, T17, T18, T19, T20