DML Analysis: How to get GOterms

Gene Set Enrichment Analysis Workflow:

  • Get Entrez Gene IDs
  • Match IDs with GOterms
  • Use both topGO and DAVID for enrichment


  • The gene IDs found in the C. virginica GFF files are not official, NCBI Entrez Gene IDs. Not sure what LOC{} is, but XM_{} are Genbank IDs. Genbank IDs from the GFF were not recognized by DAVID


  • blastx to get Uniprot accession codes and GOterms
  • Use Uniprot and GOterms in DAVID
  • Convert Uniprot accession codes to Entrez IDs
  • Use Entrez IDs and GOterms in DAVID