DML Analysis: How to get GOterms

Gene Set Enrichment Analysis Workflow:

• Get Entrez Gene IDs
• Match IDs with GOterms
• Use both topGO and DAVID for enrichment

Problem:

• The gene IDs found in the C. virginica GFF files are not official, NCBI Entrez Gene IDs. Not sure what LOC{} is, but XM_{} are Genbank IDs. Genbank IDs from the GFF were not recognized by DAVID

Solution:

• blastx to get Uniprot accession codes and GOterms
• Use Uniprot and GOterms in DAVID
• Convert Uniprot accession codes to Entrez IDs
• Use Entrez IDs and GOterms in DAVID